Wet lab/cell culture protocols

• Buffer S recipe (PDF)
• Download Cell Transformation Protocol (PDF)
• Freezing Cells Protocol (PDF)
• Passing Cells Protocol (PDF)
• PEG Chambers Protocol (PDF)
• Protein Pulldown on Dynabeads for MS Protocol (PDF)
• Standard STORM Buffer Protocol (PDF)
• Thawing Cells Protocol (PDF)
• Trypsin EDTA Solution Protocol (PDF)
• Lentiviral Transduction Protocol (PDF)
• Fibronectin Dish Coating Protocol (PDF)
• PEG Chambers Protocol (PDF)

Microscope, Laser et.al Protocols and Manuals

• Mai Tai Manual (PDF)
• Pockels Cell Transmission and Extinction Ratios in the NIR(PDF)
• Tsunami Alignment Tips (for old times' sake) (PDF)
• Eppendorf 5242 Microinjector Manual (PDF)

Wet Lab Maintenance Protocols and Manuals

• pH Meter Calibration Protocol (PDF)
• 1M HCl/NaOH Solution Preparation Protocol (PDF)
• Cleaning Hot Water Bath Protocol (PDF)
• Cleaning Tissue Culture Hood Protocol (PDF)
• Incubator Maintenance and Calibration Protocol (PDF)
• Phosphate Buffered Solution (9L) Protocol (PDF)
• Nanopure Manual (PDF)
• Nanopure Manual 2 (PDF)