Spectroscopy
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We have some basic spectroscopy instrumentation in our lab that we share with
the Cornell community for the measurement of absorption, fluorescence,
fluorescence anisotropy, and time-resolved fluorescence. Cary 300 absorption UV-Vis spectrophotometer - measures absorption spectra from 190-900 nm. It also has custom holders for measuring filter and dichroic transmission spectra. PTI QuantMaster QM-4 fluorimeter - measures fluorescence emission and excitation spectra, steady-state fluorescence time courses and polarization anisotropy. (Link to the PTI Felix GX Software Manual) We also have time-correlated single photon counting (TCSPC) instrumentation for measuring fluorescence lifetimes, either for cuvette measurememts or combined with one of the Zeiss confocal/NLO systems for Fluorescene Lifetime Imaging Microscopy (FLIM). TCSPC is carried in collaboration with the Zipfel lab. For access contact Dr. Warren Zipfel (wrz2@cornell.edu) |