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An NIH-NIBIB Biomedical Technology Resource

 

 

 

Software

Fluorescence Photon Burst Analyzer

Using this software tool, stored photon count data can be analyzed to locate large bursts of photons and to quantify the size (photons per bursts), width and interval between bursts; and perform segmented FCS and FCCS (auto- and cross-correlation between located bursts in the data stream). Originally developed for DNA fragment analysis in nanochannels (Foquet et al. 2002), this software has been upgraded for use in our in vivo and in vitro protein aggregation studies.

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FCS and FCCS Acquisition Software (will be made available after hardware designs are posted)

A multi-tau software correlator for data acquired by our data acquisition cards. The current prototype buffers incoming channels of photon count data, calculates and displays the correlation results in real-time. This application requires a PCI-based data acquisition card (ADVANTECH PCI-1755) and DRBIO-built counter boards. (see hardware section).

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MPM Scanning and Acquisition Software - requires specialized hardware (see hardware section)

A control and image acquisition software for DRBIO's various multiphoton microscope systems. Written in C and C++ (Microsoft Visual Studio 2005).

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Link to development blog

 

LaserSharp 2000 Macro

A suite of programs written in Visual Basic and VBScript that extends the functionality of LaserSharp 2000 control software for the BioRad MRC-1024 system at Weill Medical College. The programs automate various image-related tasks as they communicate with an external xy-stage controller (ASI MS-2000) and the microscope. These custom programs have been written since various prepackaged scripts bundled in the LaserSharp software will not work with the ASI xy-stage controller.

The figure below shows a GUI that can be used to create a mosaic of tiled images in a number of Z-stack formations.

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Foquet, M., J. Korlach, W. Zipfel, W. W. Webb and H. G. Craighead (2002). "DNA fragment sizing by single molecule detection in submicrometer-sized closed fluidic channels." Analytical Chemistry 74(6): 1415-1422. (PMCIDISI:000174414900030)

 

 



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