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Biography
Education:
B.S. 1987 Biochemistry, Cornell University
Ph.D 1993 Biophysics, Cornell University, Thesis
title: Modeling photon capture, excitation energy transfer and charge
transfer in photosynthesis.
Research and professional experience:
1984-1987 Boyce Thompson Institute, Research Assistant.
1987-1990 Cornell University, Teaching Assistant
1990-1993 Cornell University, NIH Molecular Physics
Traineeship
1994-1996 Cornell University, Postdoctoral Research
Associate, Applied Physics
1996-1997 Cornell University, Research Associate
III, Applied Physics
1998-present Senior Research Associate, Applied
and Engineering Physics and Associate Director of the Developmental
Resource for Biophysical Imaging and Opto-Electronics, an NIH/NCRR
Resource at Cornell University.
Organizer: “Multiphoton Microscopy in the
Biomedical Sciences” SPIE Conference on Biomedical Optics,
2001-2004.
Organizer: “Advances in Quantitative Optical
Microscopy” Symposium at Microscopy & Microanalysis 2003
Organizer: “Imaging and Bio-analysis Using
Non-Linear Excitation" Symposium at OSA annual meeting 2003.
Publications:
Orth, R. N. J. Kameoka, W. Zipfel, B. Ilic, W.
W. Webb, T. G. Clark, and H. G. Craighead. “Creating Biological
Membranes on the Micron Scale: Forming Patterned Lipid Bilayers
Using a Polymer Lift-Off Technique” 2003 In press : Biophysical
Journal.
Stroh,
M, W.R. Zipfel, R.M. Williams, W.W. Webb, and W.M. Saltzman, (2003)
“Dynamic Behavior of Nerve Growth Factor in Rat Stratum,”
Biophysical. Journal 85: 581-588
Zipfel,
W.R., R. M. Williams, R. Christie, A. Y. Nikitin, B.T. Hyman, and
W. W. Webb. (2003) Live tissue intrinsic emission microscopy using
multiphoton excited native fluorescence and second harmonic generation.
PNAS 100(12), 7075-7080.
Larson,
D., W.R Zipfel, R.M. Williams, S. Clark, M. Bruchez, F. Wise and
W.W Webb, (2003) Novel water-soluble quantum dots with large two-photon
cross-sections for biological imaging. Science 300(5624):1434-6.
Echevarría,
W, M. F. Leite, M. T. Guerra, W. R. Zipfel, and M. H. Nathanson
(2003) “Regulation of calcium signals within the nucleus by
a nucleoplasmic reticulum", Nature Cell Biology. 5(5):440-446.
Ladewig,
T., Kloppenburg, P., Lalley, P. M., Zipfel, W. R., Webb, W. W.,
and Keller, Bernhard U., (2003) "Multiphoton microscopy reveals
mitochondrial regulation of calcium microdomains in motoneurones
that are particularly vulnerable during amyotrophic lateral sclerosis
(ALS)-related motoneuron disease.” Journal of Physiology, 547(3):775-787.
P.
Kloppenburg, B.R. Johnson, W.R. Zipfel, W.W. Webb, R.M. Harris-Warrick.
(2003) Multiple effects of a single neuromodulator on an identified
motoneuron revealed by combined electrophysiological and imaging
techniques.In: Frontiers in Crustacean Neurobiology. (ed. K. Wiese)
Springer Verlag, Berlin, Heidelberg, pp 204-216.
Ouzounov,
D. G., K. D. Moll, M. A. Foster, W. R. Zipfel, W. W. Webb and A.
L. Gaeta. (2002) Delivery of nanojoule femtosecond pulses through
large-core microstructured fibers. Optics Letters 27(17), 1513-1515.
Foquet,
M., J. Korlach, W.R. Zipfel, W.W. Webb and H.G. Craighead, (2002)
“DNA fragment sizing by single molecule detection in submicrometer-sized
closed fluidic channels,” Analytical Chemistry 74(6), 1415-1422.
O’Neill,
A.F., R.E. Hagar, W.R. Zipfel, M.H. Nathanson, and B.E. Ehrlich,
(2002) Regulation of the type III InsP3 receptor by InsP3 and calcium.
Biochem Biophys Res. Comm. 294: 719-725.
Bago,
B. W.R. Zipfel, R.M. Williams, J. Jun, R. Arreola, P. Lammers, P.
E. Pfeffer, and Y. Shachar-Hill , (2002) “Translocation And
Utilization Of Fungal Lipid In The Arbuscular Mycorrhizal Symbiosis,”
Plant Physiology 128, 108-124 2002
Zipfel,
W.R. and W.W. Webb, (2001) "In Vivo Diffusion Measurements
using Multiphoton Excited Fluorescence Photobleaching Recovery (MPFPR)
and Fluorescence Correlation Spectroscopy (MPFCS)," in Methods
in Cellular Imaging, Ed(s) A. Periasamy, Oxford University Press,
Oxford, UK, pp.216-235, 2001.
Williams
R, W.R. Zipfel and W.W Webb, (2001) Multiphoton Microscopy in Biological
Research. Cur Opin.Chem. Biol. 5:603-608.
Bacskai,
B.J., S. T. Kajdasz R. H. Christie, W. R. Zipfel, R. M. Williams,
K.A. Kasischke, W. W. Webb and B. T. Hyman, (2001) Chronic imaging
of amyloid plaques in the live mouse brain using multiphoton microscopy.
Proceedings of SPIE 4262:125-133.
Ouzounov,
D, D. Homoelle, W. Zipfel, W. W. Webb, A. L. Gaeta, J. A. West,
J. C. Fajardo and K. W. Koch, (2001) Dispersion measurements of
microstructured fibers using femtosecond laser pulses, Optics Comm.
192(3), 219-233.
Christie,
R.H., B.J. Bacskai, W.R. Zipfel, R.M. Williams, S.T. Kajdasz, W.W.
Webb and B.T. Hyman, (2001) “Growth arrest of individual senile
plaques in a model of Alzheimer’s disease observed by in vivo
multiphoton microscopy,” J. Neurosci. 21(3), 858–864.
Zipfel,
W.R. (2000) Review of “Focus on Multidimensional Microscopy”
vol. 1 and 2., Nature Cell Biology, 2(12): E222,
Kloppenburg,
P., W.R. Zipfel, W.W. Webb and R.M. Harris-Warrick, (2000) “Highly
Localized Ca2+ Accumulation Revealed by Multiphoton Microscopy in
an Identified Motoneuron and Its Modulation by Dopamine,” J.
Neurosci. 20(7), 2523-2533.
Bago,
B., W.R. Zipfel, R.M. Williams, and Y. Piché, (1999) "Nuclei
of symbiotic arbuscular mycorrhizal fungi, as revealed by in vivo
two-photon microscopy," Protoplasma 209 (1-2), 77-89.
Caylor,
C. L., I. Dobrianov, C. Kimmer, W. Zipfel, and R.E. Thorne (1999)
“Fluorescence Imaging of Impurity Distributions in Protein
Crystals” PRE Rapid Comm.
Williams,
R.M., J.B. Shear, W.R. Zipfel, S. Maiti and W.W. Webb, (1999) “Mucosal
mast cell secretion processes imaged using three-photon microscopy
of 5-hydroxytryptamine autofluorescence,” Biophysical Journal
76(4), 1835-1846.
Brown,
E.B., E.S. Wu, W. Zipfel and W.W. Webb, (1999) “Measurement
of molecular diffusion in solution by multiphoton fluorescence photobleaching
recovery,” Biophysical Journal 77(5), 2837-2849.
Caylor,
C.L., I. Dobrianov, C. Kimmer, R.E. Thorne, W. Zipfel and W.W. Webb,
(1999) “Two-photon fluorescence imaging of impurity distributions
in protein crystals,” Physical Review E 59(4), R3831-R3834.
Caylor,
C.L., I. Dobrianov, S.G. Lemay, C. Kimmer, S. Kriminski, K.D. Finkelstein,
W. Zipfel, W.W. Webb, B.R. Thomas, A.A. Chernov and R.E. Thorne,
(1999) “Macromolecular impurities and disorder in protein crystals,”
Proteins-Structure Function and Genetics 36(3), 270-281.
Bago,
B., W. Zipfel, R.M. Williams, H. Chamberland, J.G. Lafontaine, W.W.
Webb and Y. Piche, (1998) “In vivo studies on the nuclear behavior
of the arbuscular mycorrhizal fungus Gigaspora rosea grown under
axenic conditions,” Protoplasma 203(1-2), 1-15.
Zipfel,
W. R., R. M. Williams and W. W. Webb (1997) “Application of
Multiphoton Imaging to Study of the Vasculature”. Microscopy
and Microanalysis 3:334-335.
Kohler,
R.H., J. Cao, W.R. Zipfel, W.W. Webb and M.R. Hanson, (1997) “Exchange
of protein molecules through connections between higher plant plastids,”
Science 276(5321), 2039-2042.
Kohler,
R.H., W.R. Zipfel, W.W. Webb and M.R. Hanson, (1997) “The green
fluorescent protein as a marker to visualize plant mitochondria
in vivo,” Plant Journal 11(3), 613-621.
Maiti,
S., J.B. Shear, R.M. Williams, W.R. Zipfel and W.W. Webb, (1997)
“Measuring serotonin distribution in live cells with three-
photon excitation,” Science 275(5299), 530-532.
Xu,
C., R.M. Williams, W.R. Zipfel and W.W. Webb, (1996) “Multiphoton
Excitation Cross-Sections of Molecular Fluorophores,” Bioimaging
4(3), 198-207.
Xu,
C., W. Zipfel, J.B. Shear, R.M. Williams and W.W. Webb, (1996) “Multiphoton
fluorescence excitation: New spectral windows for biological nonlinear
microscopy,” PNAS 93(20), 10763-10768.
Laible,
PD, WR Zipfel and TG Owens. (1993). Excited state dynamics in chlorophyll-based
antennae: the role of transfer equilibrium. Biophysical Journal
, 66:844-863.
Zipfel
WR, and TG Owens. 1991. Calculation of absolute photosystem I cross-sections
from P700 photooxidation kinetics. Photosynth. Res. 29:23-35.
Lee,
JW, WR Zipfel and TG Owens. 1991 Quenching of chlorophyll excited
states in photosystem I by quinones: Stern-Volmer analysis of fluorescence
and photochemical yield. J. Luminescence. 51:79-89.
Zipfel
WR, RG. Alscher and L Zucker. 1990. Effects of sulfite on phosphoenolpyruvate
carboxylase and malate dehydrogenase in epidermal tissue. Physiologia
Plantarium 79:491-496.
Invited Talks:
June
26, 2003 “NEW DIRECTIONS AND REALIZABLE CHALLENGES IN BIOLOGICAL
IMAGING”, NCRR/NIBIB P41 Principal Investigators Meeting, Washington,
DC
May
23, 2003 “Multiphoton Microscopy: Applications in Cancer Biology”,
National Cancer Institute, Washington, DC
May
9, 2003 “Multiphoton Microscopy and Cancer Biology”, University
of Pittsburgh Cancer Institute, Cancer in the Laboratory and Clinic:
a symposium in celebration of the opening of the Hillman Cancer
Center, Pittsburgh PA.
January
28, 2003 “Advances in multiphoton imaging: instrumentation
and applications.” SPIE Conference on Biomedical Optics, San
Jose, CA. , “Multiphoton Microscopy In The Biomedical Sciences”
symposium.
November
20, 2002. “New Developments in Nonlinear Microscopy and Correlation
Spectroscopy” Wageningen University, Wageningen, Netherlands.
November
1, 2002. “Multiphoton Microscopy: Fundamentals and Applications”
Symposium on multiphoton microscopy to study cellular and intracellular
processes, American Society of Nephrology Annual Meeting.
June
23-25, 2002 “Nonlinear Microscopy and Nanotechnology: Tools
for Systems Biology”, NCRR P41 Principal Investigators Meeting,
Washington, DC
January
29, 2002 “Optics, Electronics and Imaging - Getting Data from
a Nanodevice,” Nanobiotechnology Center Technology Platform
Series, Cornell University, Ithaca, NY
January
22, 2002 “Optimizing in vivo multiphoton imaging: instrumentation
and applications.” SPIE Conference on Biomedical Optics, San
Jose, CA. , “Multiphoton Microscopy In The Biomedical Sciences”
symposium.
January
22, 2002 “An Examination of the autocorrelation function and
other algorithms for analysis of photon history traces.” SPIE
Conference on Biomedical Optics, San Jose, CA. , “Multiphoton
Microscopy In The Biomedical Sciences” symposium
October
22, 2001 "In vivo Multiphoton Microscopy". Department
of Anatomy and Structural Biology Seminar Series. Albert Einstein
College of Medicine, Bronx, NY.
May
15, 2001 “Advances in Nonlinear Microscopy. “University
of Goteborg: Advances in Bioimaging Symposium.. Goteborg, Sweden.
January
21, 2001 , “Multiphoton microscopy and related technologies:
Lessons from a decade of applications” Invited talk at the
International Conference on Biomedical Optics San Jose, CA. , “Multiphoton
Microscopy In The Biomedical Sciences” symposium.
October
23, 2000 "GFP Multiphoton Imaging and Correlation Fluorescence
Spectroscopy," Cold Spring Harbor Laboratory Colloquium, Cold
Spring Harbor, NY
Dec.
12, 2000. “Vital Imaging Techniques in 2-photon Microscopy:
Recent Advances in Probe and Instrument Development” American
Society for Cell Biology Annual Meeting. San Francisco CA,
Dec.
13, 1999. “Imaging and non-imaging uses of Multiphoton excitation
in Cell Biology” Advances in Confocal Microscopy and Multiphoton
Imaging Symposium at the American Society for Cell Biology Meeting,
Washington DC.
August
5, 1999. “Applications of Multiphoton Microscopy ” 3nd
Annual MSA Workshop on Multiphoton Microscopy, Microscopy and Microanalysis
’99, Portland, OR.
March
19, 1999 "Multiphoton laser scanning fluorescence microscopy:
the technique and its application", Oxford University, Oxford
England.
March
15, 1999 "Multiphoton laser scanning fluorescence microscopy:
the technique and its application", German Cell Biology Meeting,
Rostock, Germany.
October
15, 1998 "Multiphoton fluorescence microscopy of cells and
tissues." 25th annual conference of the Federation of Analytical
Chemistry and Spectroscopy Societies, Austin, TX.
August
7-9, 1998 “Imaging Structure and Functions in the Nervous System”
Cold Spring Harbor Laboratories, Cold Spring Harbor, NY.
July
11, 1998 “Multiphoton Imaging in Highly Scattering Samples,”
Multiphoton Microscopy Satellite Meeting, Annual Meeting of the
Microscopy Society of America, Atlanta, GA.
June
2, 1998: Warren Zipfel: “Application of Multiphoton Microscopy
in Neuroscience,” UCLA Brain Research Institute, University
of California at Los Angeles.
April
25, 1997 “Multiphoton Microscopy Training Course,” Bio-Rad,
Hercules, CA.
February
28, 1998: “Multiphoton Microscopy: Fundamental principles,
advantages and disadvantages” Bio-Rad, San Francisco, CA
November
13, 1997 “Multiphoton excitation imaging and photochemistry
in cells and tissue,” Advances in Cellular Imaging for Biological
Research and Drug Development, San Diego, CA.
November
11, 1997 “Application of Multiphoton Microscopy,” Scripps
Institute, La Jolla, CA.
October
3, 1997 “Applications of Multiphoton excitation imaging in
the Plant Sciences,” SR Noble Foundation, Ardmore, OK
August
11, 1997 “Biological Applications of Multi-Photon Excitation
Fluorescence Imaging,” Microscopy and Microanalysis ‘97,
Cleveland, OH.
August
9, 1997: “Multi-photon excitation of intrinsic fluorescence
in cells and intact tissue,” Applications of Multiple Photon
Excitation Imaging Symposium and Short Course, Cleveland, OH.
July
15, 1997 "Potential Applications of Multiphoton Microscopy
in Medicine" Advances in Optical Technologies for Medicine
and Surgery, Snowbird, Utah .
June
20, 1997. “Applications of Multiphoton Microscopy”, 2nd
annual 3D Microscopy of Living Cells Course, Vancouver BC, Canada.
May
14, 1997. “Using Multiphoton Microscopy - A Primer for Biologists.”
Analytical and Quantitative Light Microscopy Course, Woods Hole,
Mass.
March
5, 1997. “Multiphoton Excitation of Intrinsic Fluorescence
in Cells and Intact Tissue”. Symposium on Nonlinear Microscopy,
41st Annual Meeting of the Biophysical Society, New Orleans, LA.,
August
6, 1996: “Principles of Two-Photon Microscopy,” Cold Spring
Harbor Laboratory, Cold Spring Harbor, NY.
Published Abstracts:
Zipfel,
W.R., J. Korlach, L. Kwok, R.M. Williams, W.W. Webb and L. Pollack.
(2003) “Binding kinetics in a laminar flow mixing device: modeling
and experimental verification” Biophysical Society 47th Annual
Meeting, San Antonio, Biophysical Journal, 312a.
Williams,
R.M., W.R. Zipfel, and W.W. Webb. (2003) “Multiphoton Anisotropy
imaging for obtaining spatially-resolved molecular orientation and
dynamics.” Biophysical Society 47th Annual Meeting, San Antonio,
Biophysical Journal, 285a.
Larson,
D.R., W.R. Zipfel, R.M.Williams, S., Clark, M. Bruchez, F.Wise and
W.W. Webb. (2003) Novel Water-Soluble Quantum Dots With Large Two-Photon
Cross-Sections for Biological Imaging. Biophysical Society 47th
Annual Meeting, San Antonio, Biophysical Journal, 23a.
Orth,
R. N., W. Zipfel, M.Wu, J. Kameoka, T. G. Clark, D. Holowka, B.
Baird, and H. G. Craighead. (2002) “Patterned Biomimetic Supported
Lipid Bilayers” American Society for Cell Biology Annual Meeting,
San Francisco, CA
Zipfel,
W. R., D. G. Ouzounov, A. L. Gaeta and W. W. Webb. (2002) "The
use of air-silica microstructured optical fibers for delivery of
femtosecond pulses in the near IR," Biophysical Society 46th
Annual Meeting, San Francisco, Biophysical Journal, 2426a.
Williams,
R. M., W. R. Zipfel and W. W. Webb. (2002) "Second harmonic
imaging for collagen structure analysis," Biophysical Society
46th Annual Meeting, San Francisco, Biophysical Journal, 856a.
Korlach,
J., M. Foquet, W. Zipfel, W. W. Webb and H. G. Craighead. (2002)
"DNA fragment sizing by single molecule detection in submicrometer-sized
closed fluidic channels," Biophysical Society 46th Annual Meeting,
San Francisco, Biophysical Journal, 1757a.
Korlach,
J., L. Kwok, S. A. Kim, M. N. Waxham, W. Zipfel, W. W. Webb and
L. Pollack. (2002) "Measurement of rapid conformational changes
of proteins in a fast laminar flow mixing device," Biophysical
Society 46th Annual Meeting, San Francisco, Biophysical Journal,
117a.
Kloppenburg,P,
Zipfel,W.R., Webb,W.W. and R.M. Harris-Warrick. (2002) “Differential
modulation of voltage-activated Ca2+ accumulation in identified
motoneurons.” Zoology-Jena.:105 (Sup. 5): 67. 95th Annual Meeting
of the Deutsche Zoologische Gesellschaft.
Keller,
B. U., T. Ladewig, P. Kloppenburg, W. W. Webb and W. R. Zipfel.
(2002) "Intracellular Ca release in motoneurons that are selectively
vulnerable in a mouse model of human amyotrophic lateral sclerosis
as revealed by multiphoton microscopy," Biophysical Society
46th Annual Meeting, San Francisco, Biophysical Journal, 3169a.
Lai,
G, Kloppenburg, P; Zipfel, W.R., Webb, W.W.; McCobb,D.P., (2002)
Regulation of Alternative Splicing Of Slo Potassium Channels In
Bovine Chromaffin Cells By Ca2+. Society for Neuroscience Abstract,
32nd Annual Meeting of the Society for Neuroscience, Orlando, Florida,
USA, November 02-07, 2002.
Zipfel,
W. (2002) "Optimizing in-vivo multiphoton imaging: instrumentation
and applications," Proceedings of SPIE, 23
Zipfel,
W. (2002) "Examination of the autocorrelation function and
other algorithms for analysis of photon history traces," Proceedings
of SPIE, 53.
Choi,
K.-C., R. M. Williams, W. R. Zipfel, W. W. Webb and A. Y. Nikitin.
(2002) "Live imaging of the mouse ovary by multiphoton microscopy,"
Ovary Roundtable at the Mouse Models of Human Cancers Consortium
(NIH/NCI) Steering Committee Meeting, Washington, DC, Abstracts
of Ovary Roundtable at the Mouse Models of Human Cancers Consortium
(NIH/NCI) Steering Committee Meeting.
Zipfel,
W.R., S.A. Kim, M.N. Waxham and W.W. Webb, (2001) "Techniques
for quantification of molecular mobility in cells illustrated by
measurements of calmodulin diffusion in neurons," Biophysical
Journal 80(1), 2777a.
Kloppenburg,
P., W.R. Zipfel, W.W. Webb and R.M. Harris-Warrick, (2001) "Differential
modulation of voltage-activated Ca2+ accumulation in identified
motoneurons revealed by multiphoton microscopy," Biophysical
Journal 80(1), 945a.
Kloppenburg,P,
Zipfel, W.R, Lalley, P.M., Webb, W.W; and B.U. Keller. (2001) “Intracellular
calcium release in motoneurons that are selectively vulnerable in
a mouse model of human amyotrophic lateral sclerosis as revealed
by multiphoton microscopy”. Pfluegers-Archiv-European-Journal-of-Physiology.
441 (6 Supplement): R162. Joint Congress of the Scandinavian and
the German Physiological Societies, Berlin, Germany,
Kim,
S.A., Zipfel, W.R., Schwille, P. Webb, W.W. and M.N. “Calmodulin
availablity in neuronal compartments.”: Society-for-Neuroscience-Abstracts
27 (2): 1799.
Kloppenburg,
P., W.R. Zipfel, W.W. Webb, and R.M. Harris-Warrick, (2000) “Modulation
of Localized Ca2+ Accumulation in Identified Motoneurons Revealed
by Multiphoton Microscopy,” Society for Neuroscience 26, 335.
Ladewig,
T., P. Kloppenburg, W.R. Zipfel, W.W. Webb and B.U. Keller, (2000)
"Intracellular calcium release in hypoglossal motoneurons monitored
by multiphoton microscopy," Eur. J. Neurosci. 12, 226-226.
Zipfel,
W.R., S.A. Kim, M.N. Waxham, and W.W. Webb, (2000) "Local Diffusion
of Calmodulin in Neurons Measured Using Multiphoton Fluorescence
Photobleaching Recovery (MPFPR) and Fluorescence Correlation Spectroscopy
(MPFCS)," Biophysical Journal 78 (1), 2308a.
Kloppenburg,
P., W.R. Zipfel, W.W. Webb, and R.M. Harris-Warrick, (2000) "Aminergic
Modulation of Localized CA2+ Accumulation in an Identified Motoneuron
Measured by Multiphoton Microscopy," Biophysical Journal 78
(1), 2307a.
Kim,
S.A., W.R. Zipfel, P. Schwille, W.W. Webb, and M.N. Waxham, (1999)
"Assessing Local Diffusion of Calmodulin in Neurons Using Multiphoton
Fluorescence Photobleaching Recovery (MPFPR) and Fluorescence Correlation
Spectroscopy (MPFCE)," Molecular Biology of the Cell 10: 1929.
Christie,
R.H., W.R. Zipfel, R.M. Williams, W.W. Webb, and B.T. Hyman, (1999)
"In vivo multiphoton imaging of amyloid deposition in transgenic
mice," Journal of Neuropathology and Experimental Neurology
58, (5) 204.
Zipfel,
W.R., P. Kloppenburg, R.M. Harris-Warrick, and W.W. Webb, (1999)
“Modulation of calcium influx into the neurties of identified
motor neurons monitored during voltage clamp by multiphoton microscopy,”
Biophysical Journal 76 (1 PART 2), A21.
Caylor,
C.L, C. Kimmer, I. Dobrianov, K.D. Finkelstein, W. Zipfel, W.W.
Webb and R.E. Thorne, (1999) “The spatial distribution of impurities
in protein crystals and associated impurity effects,” Biophysical
Journal 76, a350.
Zipfel,
W.R., P. Kloppenburg, R. M. Harris-Warrick, and W.W. Webb, (1999)
“Modulation of calcium influx into the neuritis of identified
motor neurons monitored during voltage clamp by multiphoton microscopy,”
Biophysical Journal, 76, a21.
Williams,
R. M., W. R. Zipfel, W. W. Webb, (1999) “ Granule ph Jumps
Preceding Exocytosis Imaged During Mucosal Mast Cell Secretion,”
Biophysical Journal, 76, A397.
Kloppenburg,
P., W.R. Zipfel, W.W. Webb and R.M. Harris-Warrick, (1998) “Ca++
influx into voltage clamped neurons of the pyloric network monitored
by multiphoton excitation microscopy,” 28th Annual Meeting
of the Society for Neuroscience, Session: 752.5; Circuitry and Pattern
Generation; Board number: Q-3.
P.
Kloppenburg, W. R. Zipfel, W.W. Webb and R.M. Harris-Warrick, (1998)
“Localized Ca++ Accumulation in an Identified Motoneuron Monitored
by Two-Photon Excitation Microscopy,” German Society of Neuroscience,
R.H.
Christie, W.R. Zipfel, R.M. Williams, W.W. Webb, and B.T. Hyman,
(1998) “Multiphoton imaging of Alzheimer's disease neuropathology,”
Society for Neuroscience Abstracts 24 (1-2), Page 1219.
Kohler,
R.H., J. Cao, W.R. Zipfel, W.W. Webb, and M.R. Hanson, (1998) “Interplasmid
communications in higher plants,” Cytometry 27 (SUPPL. 9).
Montrose,
M.H., S. Chu, W.R. Zipfel, W.W. Webb (1997) “Advantages of
2-photon versus confocal microscopy for imaging pH or 5-aminosalicylate
(5-ASA) in living colonic mucosa” Gastroenterology 112 (4),
A387
Zipfel,
W. R., R. M. Williams, and W. W. Webb, (1997) “Application
of multiphoton imaging to study of the vasculature,” Microscopy
and Microanalysis, Volume 3, Supplement 2, pp.334-335.
Rebecca
M. Williams, Jason B. Shear, Warren R. Zipfel, Sudipta Maiti and
Watt W. Webb, (1997) “Three-Photon Excitation Imaging of Serotonin
Secretion by RBL-2H3 Cells,” Biophysical Journal 72, TU100.
Xu,
Chris, Warren Zipfel and Watt W. Webb, (1996) “Three-Photon
Excited Fluorescence and Applications in Nonlinear Laser Scanning
Microscopy,” Biophysical Journal 70, WP297.
Zipfel,
Warren R., James P. O’Malley, Dirk Van Helden, Rebecca M. Williams,
Jeffrey B. Guild, Miriam M. Salpeter, and Watt W. Webb, (1996) “Characterization
of Spontaneous Calcium Waves and Sparks in Primary Cultures of Fetal
Rat Myotubes Using Two Photon Excitation Point and Line Scanning
Microscopy,” Biophysical Journal 70, WP283.
Zipfel,
Warren R., Rebecca M. Williams and Watt W. Webb, (1996) “Release
of Caged Bioeffector Molecules by Two Photon Excitation: Excitation
Spectra, Absorption Cross-Sections and Practicalities of Some Common
Groups,” Biophysical Journal 70, MP248.
Zipfel,
W.R., K. Hodgson, C. Conley and W.W. Webb, (1995) “Thick, Cloudy
Sample Fluorescence Imaging Characteristics: Comparison Between
One and Two Photon Excitation”, Biophysical Journal 68, A290.
Patents:
Bradley
T. Hyman, Richard Christie, Brian Bacskai, Watt W. Webb, and Warren
R. Zipfel, "In Vivo Alzheimer's Disease Diagnostic Using Multiphoton
Microscopy," U.S. Patent Provisional Filing (2000)
Research support:
NIH,
R33 CA094311-01 ($1.65 M over 3 years) 6/1/02-5/31/05
“Nonlinear
Intrinsic Microspectroscopy of Cancer”
The
major goal of this research is to utilize the unique advantages
of multiphoton fluorescence excitation and nonlinear scattering
to develop methods for in vivo detection of cancer and in vivo studies
in cancer biology.
Role on Project: PI
NIH-NCRR,
P41-RR04224 (Watt W. Webb – PI, ~$5M over 5 years) August 1999-September
2003
“Biotechnology Research for Digital Electro-Optical Imaging”
– Developmental Resource for Biophysical Imaging and Optoelectronics.
The major goals of this project are to provide facilities in advanced
imaging technologies for exploratory and collaborative biological
and biomedical research. Core research creates and develops new
technologies for visualization and measurement of molecular mechanisms
of dynamic cellular processes. Methods are applied through collaborative
research and use of facilities and disseminated through exploratory
experiments, consultation and pedagogical publications.
Role on Project: Associate Director of Resource
BioRad Microscience, Ltd. ($50K/year) 1996-2003
Multiphoton Microscope Development
Role on project: Co-PI/consultant
GlaxoSmithKline
Inc. ($250K) 2002-2003
Investigations on the use of nonlinear microscopy and fluorescence
correlation methods for drug discovery.
Role on Project: Co-PI/consultant.
Pending:
U-01
(Co-PI) Mouse models of Human Cancers Consortium 4/1/04 – 3/31/2010.
$93,721 (first year).
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